A semi-simulated dataset for testing and demonstrating flowthrough respirometry analyses. Contains one column of numeric time data (col 1 in mins), four columns of outflow oxygen concentrations (cols 2:5), four columns of inflow oxygen concentrations (cols 6:9), and four columns of delta oxygen concentrations (cols 10:13, which is simply the numeric difference between paired columns of outflow and inflow). There is also a column of inflow oxygen concentrations as recorded from a shared header tank (col 14, $oxy.header) supplying all chambers, to use as an alternative to the individual inflow oxygen recordings. Lastly, there is a column of temperature data (col 15, $temperature in °C).

flowthrough_mult.rd

Format

A data frame object consisting of 3740 rows (approx 62 mins of data),and 15 columns: time (col 1), oxygen outflow concentrations (cols 2,3,4,5), inflow concentrations (cols 6,7,8,9 each paired with the respective outflow column, the fourth being a control), delta oxygen values (cols 10,11,12,13 or difference between outflow and inflow concentrations), inflow concentrations recorded in a shared header tank (col 14), and temperature (col 15).

Details

Outflow (2:5) and inflow (6:9) columns are paired, with the first three containing specimens, and the fourth an empty control respirometer, or "blank" experiment (oxy.out.blank, oxy.in.blank) to determine background respiration.

The third paired dataset (col 4 and col 8 pair) has a period of higher rates at around the 40 minute timepoint, where the specimen increases its activity then slowly recovers to routine respiration levels.

  • Dissolved oxygen units: %Air

  • Time units: mins

  • Flow rate (L/min): 0.1

  • Specimen masses: (kg): 0.013, 0.015, 0.020

  • Mean temperature (°C): t = 18

  • Salinity: S = 0, i.e. freshwater

  • Atmospheric pressure (bar): P = 1.013

Author

Nicholas Carey